Genomic Analysis of Epigenetic Regulation
We leverage the strength of ATAC-seq to profile open chromatin landscape with as few as 50 thousand cells, and have
optimized an "on-plate" protocol for adherent cells without the need of trypsinization. We further integrate the data analysis
of ATAC-seq, ChIP-seq, and RNA-seq to elucidate gene regulation mechanism.
Using 3'READS+ (3' Region Extraction and Deep Sequencing plus), we have recently mapped the transcriptome-wide termination sites in primary human keratinocytes. In particular, 2739 of these termination sites are mapped to introns! We are currently characterizing the function and regulation of these intronic sites.
We uncover novel protein-protein interactions, both stable and transient associations, via both conventional affinity purification as well as the BirA purification system. We are also interested in deciphering the roles of post-translational modifications such as phosphorylation and methylation.
Proteomic Identification of Interactome and Modifications
Rapid Genetic Characterization in Intact Human Tissue
Our characterization of gene function within 3-dimensional organotypic human tissue can be achieved in less than a week. We have further developed a "Stem Cell Competition Assay" to label and track living human cells in vivo by grafting self-renewing human epidermal tissue.
Organotypic Human Tissue Regeneration (~ 1 week)
Gene Regulation of Stem Cell Maintenance and Differentiation
Stem Cell Competition Assay in vivo