Genomic Analysis of Epigenetic Regulation
We uncover novel protein-protein interactions, both stable and transient associations, via both conventional affinity purification as well as the BirA purification system. We are also interested in deciphering the roles of post-translational modifications such as phosphorylation and methylation.
Organotypic Human Tissue Regeneration (~ 1 week)
Proteomic Identification of Interactome and Modifications
Rapid Genetic Characterization in Intact Human Tissue
Stem Cell Competition Assay in vivo
We leverage the strength of ATAC-seq to profile open chromatin landscape with as few as 50 thousand cells, and have
optimized an "on-plate" protocol for adherent cells without the need of trypsinization. We further integrate the data analysis
of ATAC-seq, ChIP-seq, and RNA-seq to elucidate gene regulation mechanism.
Our characterization of gene function within 3-dimensional organotypic human tissue can be achieved in less than a week. We have further developed a "Stem Cell Competition Assay" to label and track living human cells in vivo by grafting self-renewing human epidermal tissue.
Epigenomic Regulation of Stem Cell Maintenance and Differentiation